Hotspot

trnL-UAA-trnF-GAA

LSC · 48570-48939 · 370 bp

Back to Vandellia

Pi 0.064

MarkerSeek score 75.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 22
pi: 0.064
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	GACTTGAACTGGTGACACGA	50.0	50.0	57.5	57.8	442-463 bp	1.0	82.3
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	ACTTGAACTGGTGACACGAG	50.0	50.0	57.5	57.8	441-462 bp	1.0	82.3
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	AGACTTGAACTGGTGACACG	50.0	50.0	57.5	57.8	443-464 bp	1.0	82.3
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	402-423 bp	1.0	80.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	437-458 bp	1.0	80.5