Hotspot

trnG-GCC-trnfM-CAU

LSC · 37019-37192 · 174 bp

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Pi 0.0778
MarkerSeek score 80.10
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
174
variable_sites
13
pi
0.0778
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 239-240 bp 1.0 85.9
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 240-241 bp 1.0 85.9
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 233-234 bp 1.0 85.5
trnG-GCC-trnfM-CAU_p4 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 275-276 bp 1.0 85.1
trnG-GCC-trnfM-CAU_p5 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 276-277 bp 1.0 85.1