Hotspot

rpoB-trnC-GCA

LSC · 27086-28303 · 1218 bp

Back to Vandellia

Pi 0.0358

MarkerSeek score 74.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 43
pi: 0.0358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCTGTCAAACCCTGA	CCGGATTTGAACTGGGGAAA	42.9	50.0	56.8	58.1	1360-1378 bp	1.0	65.2
rpoB-trnC-GCA_p2	TCAAACCCTGATCAATGAACCT	CCGGATTTGAACTGGGGAAA	40.9	50.0	57.6	58.1	1350-1368 bp	1.0	63.8
rpoB-trnC-GCA_p3	TCTTCTGTCAAACCCTGATCA	CCGGATTTGAACTGGGGAAA	42.9	50.0	56.6	58.1	1357-1375 bp	1.0	63.5
rpoB-trnC-GCA_p4	GTCAAACCCTGATCAATGAACC	CCGGATTTGAACTGGGGAAA	45.5	50.0	57.6	58.1	1351-1369 bp	1.0	63.4
rpoB-trnC-GCA_p5	AGTTCTTCTGTCAAACCCTGA	AAAAGGATTTGCAGTCCCCC	42.9	50.0	56.8	58.4	1342-1360 bp	1.0	63.1