Hotspot

psbI-trnS-GCU

LSC · 8648-8772 · 125 bp

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Pi 0.0783

MarkerSeek score 81.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 9
pi: 0.0783
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	228-238 bp	1.0	86.0
psbI-trnS-GCU_p2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	229-239 bp	1.0	85.9
psbI-trnS-GCU_p3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	229-239 bp	1.0	85.9
psbI-trnS-GCU_p4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.1	58.0	230-240 bp	1.0	85.9
psbI-trnS-GCU_p5	ATCTAATGATCCGGGGCGTA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.1	58.1	247-257 bp	1.0	84.6