Hotspot

trnK-UUU-rps16

LSC · 4506-5371 · 866 bp

Back to Vandellia

Pi 0.0508
MarkerSeek score 77.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
866
variable_sites
43
pi
0.0508
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGCTCAACCCACAGGAAC 50.0 61.1 58.2 57.9 975-1048 bp 1.0 67.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGCTCAACCCACAGGAAC 50.0 61.1 58.2 57.9 974-1047 bp 1.0 67.0
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA GGCTCAACCCACAGGAAC 50.0 61.1 58.3 57.9 984-1057 bp 1.0 66.0
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GGCTCAACCCACAGGAACT 50.0 57.9 58.2 59.5 975-1048 bp 1.0 63.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGCTCAACCCACAGGAACT 50.0 57.9 58.2 59.5 974-1047 bp 1.0 63.3