Hotspot

trnH-GUG-psbA

LSC · 89-663 · 575 bp

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Pi 0.0827

MarkerSeek score 78.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 575
variable_sites: 41
pi: 0.0827
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	964-1024 bp	1.0	86.4
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	963-1023 bp	1.0	86.4
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	978-1038 bp	1.0	86.3
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	977-1037 bp	1.0	86.3
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	979-1039 bp	1.0	86.3