primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	89	663	964-1024	1.000000	86.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.177364	89	663	963-1023	1.000000	86.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.175967	89	663	978-1038	1.000000	86.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	89	663	977-1037	1.000000	86.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	89	663	979-1039	1.000000	86.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGCTCAACCCACAGGAAC	20	18	50.0	61.1	58.2	57.9	20.3	0.0	2.249470	4506	5371	975-1048	1.000000	67.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGCTCAACCCACAGGAAC	20	18	50.0	61.1	58.2	57.9	20.3	0.0	2.249470	4506	5371	974-1047	1.000000	67.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	GGCTCAACCCACAGGAAC	20	18	50.0	61.1	58.3	57.9	5.3	0.0	2.363856	4506	5371	984-1057	1.000000	66.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	GGCTCAACCCACAGGAACT	20	19	50.0	57.9	58.2	59.5	20.3	0.0	2.712133	4506	5371	975-1048	1.000000	63.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GGCTCAACCCACAGGAACT	20	19	50.0	57.9	58.2	59.5	20.3	0.0	2.712133	4506	5371	974-1047	1.000000	63.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6478	7549	1145-1256	1.000000	83.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6478	7549	1159-1270	1.000000	81.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6478	7549	1149-1260	1.000000	78.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6478	7549	1175-1286	1.000000	78.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6478	7549	1137-1248	1.000000	78.0
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	8648	8772	228-238	1.000000	86.0
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	8648	8772	229-239	1.000000	85.9
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072949	8648	8772	229-239	1.000000	85.9
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072949	8648	8772	230-240	1.000000	85.9
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	ATCTAATGATCCGGGGCGTA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.176426	8648	8772	247-257	1.000000	84.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AGTTCTTCTGTCAAACCCTGA	CCGGATTTGAACTGGGGAAA	21	20	42.9	50.0	56.8	58.1	0.0	0.0	2.296250	27086	28303	1360-1378	1.000000	65.2
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCAAACCCTGATCAATGAACCT	CCGGATTTGAACTGGGGAAA	22	20	40.9	50.0	57.6	58.1	0.0	0.0	2.469154	27086	28303	1350-1368	1.000000	63.8
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCTTCTGTCAAACCCTGATCA	CCGGATTTGAACTGGGGAAA	21	20	42.9	50.0	56.6	58.1	0.0	0.0	2.507733	27086	28303	1357-1375	1.000000	63.5
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GTCAAACCCTGATCAATGAACC	CCGGATTTGAACTGGGGAAA	22	20	45.5	50.0	57.6	58.1	0.0	0.0	2.522967	27086	28303	1351-1369	1.000000	63.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AGTTCTTCTGTCAAACCCTGA	AAAAGGATTTGCAGTCCCCC	21	20	42.9	50.0	56.8	58.4	0.0	14.6	2.569613	27086	28303	1342-1360	1.000000	63.1
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28375	29236	950-972	1.000000	84.9
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	28375	29236	948-970	1.000000	84.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTTGGATCATTTTGGCGGC	ACTTCTTCCCCACACTACGA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.097222	28375	29236	1006-1028	1.000000	84.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTTGGATCATTTTGGCGGC	TTCTTCCCCACACTACGAGT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.097222	28375	29236	1004-1026	1.000000	84.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.162414	28375	29236	951-973	1.000000	84.3
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	ACGTAAAAACAGTCAGTCAA	20	20	50.0	35.0	58.0	53.0	0.0	0.0	4.970112	29327	30325	1042-1059	1.000000	47.0
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	ACGTAAAAACAGTCAGTCAA	20	20	50.0	35.0	58.0	53.0	0.0	0.0	4.970112	29327	30325	1040-1057	1.000000	47.0
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	ACGTAAAAACAGTCAGTCAA	20	20	55.0	35.0	57.9	53.0	0.0	0.0	5.045028	29327	30325	1039-1056	1.000000	46.8
petN-psbM_p4	petN-psbM	4	ACTCGTAGTGTGGGGAAGAA	ACGTAAAAACAGTCAGTCAAA	20	21	50.0	33.3	58.0	53.9	0.0	0.0	5.067998	29327	30325	1042-1059	1.000000	46.8
petN-psbM_p5	petN-psbM	5	TCGTAGTGTGGGGAAGAAGT	ACGTAAAAACAGTCAGTCAAA	20	21	50.0	33.3	58.0	53.9	0.0	0.0	5.067998	29327	30325	1040-1057	1.000000	46.8
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	37019	37192	239-240	1.000000	85.9
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	37019	37192	240-241	1.000000	85.9
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.166466	37019	37192	233-234	1.000000	85.5
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	37019	37192	275-276	1.000000	85.1
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.084264	37019	37192	276-277	1.000000	85.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	47317	47988	733-766	1.000000	81.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	47317	47988	765-798	1.000000	80.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	33.1	10.2	0.482748	47317	47988	784-817	1.000000	80.2
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	47317	47988	727-760	1.000000	80.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	47317	47988	765-798	1.000000	79.1
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	GACTTGAACTGGTGACACGA	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.693363	48570	48939	442-463	1.000000	82.3
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	ACTTGAACTGGTGACACGAG	20	20	50.0	50.0	57.5	57.8	0.0	15.3	0.699478	48570	48939	441-462	1.000000	82.3
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	AGACTTGAACTGGTGACACG	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.699478	48570	48939	443-464	1.000000	82.3
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	20	20	50.0	50.0	57.5	57.6	0.0	0.0	0.921818	48570	48939	402-423	1.000000	80.9
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.5	57.5	0.0	20.1	0.931776	48570	48939	437-458	1.000000	80.5
rpl36-infA_p1	rpl36-infA	1	CTATAATACGCCCCCGCCTA	ACGCATACTGCCAGGAGATA	20	20	55.0	50.0	58.5	58.3	0.0	0.0	0.817806	80372	80469	268-274	1.000000	76.7
rpl36-infA_p2	rpl36-infA	2	CTATAATACGCCCCCGCCTA	ATACGCATACTGCCAGGAGA	20	20	55.0	50.0	58.5	58.3	0.0	0.0	0.817806	80372	80469	270-276	1.000000	76.7
rpl36-infA_p3	rpl36-infA	3	CTATAATACGCCCCCGCCTA	TACGCATACTGCCAGGAGAT	20	20	55.0	50.0	58.5	58.3	0.0	0.0	0.817806	80372	80469	269-275	1.000000	76.7
rpl36-infA_p4	rpl36-infA	4	TATAATACGCCCCCGCCTAC	ACGCATACTGCCAGGAGATA	20	20	55.0	50.0	58.8	58.3	0.0	0.0	1.110044	80372	80469	267-273	1.000000	74.4
rpl36-infA_p5	rpl36-infA	5	TATAATACGCCCCCGCCTAC	TACGCATACTGCCAGGAGAT	20	20	55.0	50.0	58.8	58.3	0.0	0.0	1.110044	80372	80469	268-274	1.000000	74.4
ndhF_p1	ndhF	1	AATACCCCTTTCGGATCGGA	GCCCAGTACCCCTTTCTTTT	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.506901	109409	111667	2921-2921	0.500000	55.9
ndhF_p2	ndhF	2	ATACCCCTTTCGGATCGGAA	GCCCAGTACCCCTTTCTTTT	20	20	50.0	50.0	58.2	57.7	11.4	0.0	0.506901	109409	111667	2920-2920	0.500000	55.9
ndhF_p3	ndhF	3	TACCCCTTTCGGATCGGAAT	GCCCAGTACCCCTTTCTTTT	20	20	50.0	50.0	58.2	57.7	15.6	0.0	0.506901	109409	111667	2919-2919	0.500000	55.9
ndhF_p4	ndhF	4	CCCTTTCGGATCGGAATTCA	GCCCAGTACCCCTTTCTTTT	20	20	50.0	50.0	57.7	57.7	15.6	0.0	0.629804	109409	111667	2916-2916	0.500000	55.0
ndhF_p5	ndhF	5	TACCCCTTTCGGATCGGAAT	TTTTAACGCTGCCCAGTACC	20	20	50.0	50.0	58.2	58.5	15.6	0.0	0.676739	109409	111667	2929-2929	0.500000	54.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGTTTCTTTGTAGGACAAACA	AGCGTGTCTACCGATTTCAC	21	20	33.3	50.0	53.2	58.0	0.7	0.0	5.807481	112353	112835	557-911	1.000000	44.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGTTTCTTTGTAGGACAAACA	CAGCGTGTCTACCGATTTCA	21	20	33.3	50.0	53.2	58.0	0.7	0.0	5.808852	112353	112835	558-912	1.000000	44.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGTTTCTTTGTAGGACAAACA	AGCGTGTCTACCGATTTCAC	22	20	31.8	50.0	54.1	58.0	0.7	0.0	5.938235	112353	112835	558-912	1.000000	44.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGTTTCTTTGTAGGACAAACAA	AGCGTGTCTACCGATTTCAC	22	20	31.8	50.0	54.1	58.0	0.7	0.0	5.938235	112353	112835	557-911	1.000000	44.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGTTTCTTTGTAGGACAAACA	TCCTAAGAGCAGCGTGTCTA	21	20	33.3	50.0	53.2	57.9	0.7	0.0	5.923928	112353	112835	567-921	1.000000	44.7
ccsA-ndhD_p1	ccsA-ndhD	1	GGGGTCAATCTATTAGGAATAGGAC	GGTTGAAGTTATTCTATCTAATTCT	25	25	44.0	28.0	58.1	52.7	6.4	0.0	15.382289	113989	114213	314-325	1.000000	45.8
ccsA-ndhD_p2	ccsA-ndhD	2	TGGGGTCAATCTATTAGGAATAGG	GGTTGAAGTTATTCTATCTAATTCT	24	25	41.7	28.0	57.0	52.7	6.4	0.0	15.283483	113989	114213	315-326	1.000000	45.7
ccsA-ndhD_p3	ccsA-ndhD	3	TGGATATGTTATTTTGGGGTCA	GGTTGAAGTTATTCTATCTAATTCT	22	25	36.4	28.0	55.1	52.7	0.0	0.0	15.232042	113989	114213	329-340	1.000000	45.5
ccsA-ndhD_p4	ccsA-ndhD	4	TGGATATGTTATTTTGGGGTCAA	GGTTGAAGTTATTCTATCTAATTCT	23	25	34.8	28.0	55.9	52.7	0.0	0.0	15.469233	113989	114213	329-340	1.000000	45.5
ccsA-ndhD_p5	ccsA-ndhD	5	TTGGATATGTTATTTTGGGGTCA	GGTTGAAGTTATTCTATCTAATTCT	23	25	34.8	28.0	55.9	52.7	0.0	0.0	15.469233	113989	114213	330-341	1.000000	45.5
ndhD_p1	ndhD	1	TTGGAGTTGGGACCCAAAAG	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	57.9	58.0	27.9	22.2	0.086024	114214	115716	2221-2233	1.000000	82.3
ndhD_p2	ndhD	2	CTAACGAAGCATGGGGATCC	GATACCCTGGGACGGATGTA	20	20	55.0	55.0	58.1	58.0	1.9	22.2	0.121135	114214	115716	2243-2255	1.000000	82.0
ndhD_p3	ndhD	3	TTGGAGTTGGGACCCAAAAG	TGTGAATCTGCCTGTCCAAC	20	20	50.0	50.0	57.9	58.1	27.9	0.0	0.184434	114214	115716	2145-2157	1.000000	81.5
ndhD_p4	ndhD	4	TTGGAGTTGGGACCCAAAAG	AAATGATACCCTGGGACGGA	20	20	50.0	50.0	57.9	58.1	27.9	22.2	0.192857	114214	115716	2225-2237	1.000000	81.4
ndhD_p5	ndhD	5	TTCAGCAATTGTGGCGTCTA	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	57.8	58.0	7.4	22.2	0.182764	114214	115716	2134-2134	0.500000	58.5
ndhD-psaC_p1	ndhD-psaC	1	TTTGCCCACTGTACATTGCT	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	58.0	58.0	7.7	22.2	0.011414	115717	115844	666-667	1.000000	84.0
ndhD-psaC_p2	ndhD-psaC	2	AACCGATACCCAGAACTCCA	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	58.1	58.0	0.0	22.2	0.058386	115717	115844	849-850	1.000000	83.1
ndhD-psaC_p3	ndhD-psaC	3	ACCGATACCCAGAACTCCAA	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	58.1	58.0	0.0	22.2	0.058386	115717	115844	848-849	1.000000	83.1
ndhD-psaC_p4	ndhD-psaC	4	CCCCCTGCTGTGTACAAAAT	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	58.1	58.0	0.0	22.2	0.082014	115717	115844	811-812	1.000000	83.1
ndhD-psaC_p5	ndhD-psaC	5	ATAATGCGCTTCTCCATGGG	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	58.1	58.0	13.0	22.2	0.103779	115717	115844	1019-1020	1.000000	82.5
ycf1_p1	ycf1	1	CATTCCTGACAGCCCTTTCA	TCGTCGTGGTTGGACTCTAT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.374743	127591	127710	1113-1113	0.500000	57.0
ycf1_p2	ycf1	2	TCCATCGCTTCGAATCGAAA	TCGTCGTGGTTGGACTCTAT	20	20	45.0	50.0	57.7	58.2	25.5	0.0	0.457114	127591	127710	1059-1059	0.500000	56.3
ycf1_p3	ycf1	3	TTCCATCGCTTCGAATCGAA	TCGTCGTGGTTGGACTCTAT	20	20	45.0	50.0	57.7	58.2	25.5	0.0	0.457114	127591	127710	1060-1060	0.500000	56.3
ycf1_p4	ycf1	4	CGGTGGATACCTCTTGTTCC	TCGTCGTGGTTGGACTCTAT	20	20	55.0	50.0	57.7	58.2	0.0	0.0	0.480845	127591	127710	780-780	0.500000	56.2
ycf1_p5	ycf1	5	AATGGGCGATGGTATTCTGC	TCGTCGTGGTTGGACTCTAT	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.560513	127591	127710	659-659	0.500000	55.5