Hotspot

rpl16

LSC · 82984-84603 · 1620 bp

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Pi 0.0191
MarkerSeek score 58.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1620
variable_sites
77
pi
0.0191
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ATTGGGCACTGCTTCTTTGA GCAAATTGCAGGACGTATCG 45.0 50.0 58.0 57.9 2209-2376 bp 1.0 86.4
rpl16_p2 TGGGCACTGCTTCTTTGATT GCAAATTGCAGGACGTATCG 45.0 50.0 58.0 57.9 2207-2374 bp 1.0 86.4
rpl16_p3 TTGGGCACTGCTTCTTTGAT GCAAATTGCAGGACGTATCG 45.0 50.0 58.0 57.9 2208-2375 bp 1.0 86.4
rpl16_p4 TGGGCACTGCTTCTTTGATT ACGGAAAAGAGATTGCACGT 45.0 45.0 58.0 58.1 2187-2354 bp 1.0 86.3
rpl16_p5 TTGGGCACTGCTTCTTTGAT ACGGAAAAGAGATTGCACGT 45.0 45.0 58.0 58.1 2188-2355 bp 1.0 86.3