Hotspot

psaC-ndhE

SSC · 115619-116233 · 615 bp

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Pi 0.0259
MarkerSeek score 63.70
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
615
variable_sites
42
pi
0.0259
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 GGACAAGCGGATTCACATCT TCACAAGTCGAAACATGGCT 50.0 45.0 58.0 57.7 1000-1067 bp 0.888889 80.9
psaC-ndhE_p2 AGCTCGAACACATTGGGTAC TCACAAGTCGAAACATGGCT 50.0 45.0 57.9 57.7 884-951 bp 0.888889 80.8
psaC-ndhE_p3 TATACCAGAAAAGCCCGTGC TCACAAGTCGAAACATGGCT 50.0 45.0 58.0 57.7 1183-1250 bp 0.888889 80.5
psaC-ndhE_p4 GGAAAGTCCATCGGTTTCCA TCACAAGTCGAAACATGGCT 50.0 45.0 57.8 57.7 1484-1533 bp 0.777778 73.6
psaC-ndhE_p5 TGGAAAGTCCATCGGTTTCC TCACAAGTCGAAACATGGCT 50.0 45.0 57.8 57.7 1485-1534 bp 0.777778 73.6