Hotspot

psbB-psbT

LSC · 75216-75674 · 459 bp

Back to Vanda

Pi 0.0256
MarkerSeek score 66.90
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
459
variable_sites
31
pi
0.0256
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.92
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 TCAGAGATGTTTTTGCCGGT TGGAACTTTAGGTGGTTCTCG 45.0 47.6 57.7 57.6 532-653 bp 1.0 74.7
psbB-psbT_p2 TCAGAGATGTTTTTGCCGGT GGAACTTTAGGTGGTTCTCGA 45.0 47.6 57.7 57.3 531-652 bp 1.0 72.5
psbB-psbT_p3 GCCAGAACCTTGTTCAGAGA TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.4 57.6 545-666 bp 1.0 72.4
psbB-psbT_p4 GCTCAAGTGGAATTTGGAGC TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.4 57.6 494-615 bp 1.0 72.0
psbB-psbT_p5 GCCAGAACCTTGTTCAGAGA GGAACTTTAGGTGGTTCTCGA 50.0 47.6 57.4 57.3 544-665 bp 1.0 70.2