Hotspot

clpP-psbB

LSC · 72949-73688 · 740 bp

Back to Vanda

Pi 0.067
MarkerSeek score 89.30
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
740
variable_sites
114
pi
0.067
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
89.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA CAACCAGAAACCAGAGCTGT 50.0 50.0 58.0 58.0 779-1092 bp 1.0 91.8
clpP-psbB_p2 CTTTTGGAACACCAATGGGC CAACCAGAAACCAGAGCTGT 50.0 50.0 57.8 58.0 725-1038 bp 1.0 90.4
clpP-psbB_p3 ACCCAAACTGCATCTTCCTC CAACCAGAAACCAGAGCTGT 50.0 50.0 57.8 58.0 765-1078 bp 1.0 90.1
clpP-psbB_p4 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 701-1014 bp 1.0 90.1
clpP-psbB_p5 TCCTCTCCAGGATTCCGAAA CAACCAGAAACCAGAGCTGT 50.0 50.0 57.8 58.0 750-1063 bp 1.0 89.8