Hotspot

atpB-rbcL

LSC · 54928-55939 · 1012 bp

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Pi 0.013
MarkerSeek score 59.70
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
1012
variable_sites
40
pi
0.013
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.93
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CGCCCTTGGTTTTGTTCTTC GGTGACATAAGTCCCTCCCT 50.0 55.0 57.9 58.1 1058-1125 bp 1.0 84.9
atpB-rbcL_p2 TGGGCCAATAATTTGAGCGA GGTGACATAAGTCCCTCCCT 45.0 55.0 57.9 58.1 1080-1147 bp 1.0 84.8
atpB-rbcL_p3 TACGCCCTTGGTTTTGTTCT GGTGACATAAGTCCCTCCCT 45.0 55.0 57.6 58.1 1060-1127 bp 1.0 83.2
atpB-rbcL_p4 CGCCCTTGGTTTTGTTCTTC TGGTGACATAAGTCCCTCCC 50.0 55.0 57.9 58.4 1059-1126 bp 1.0 82.5
atpB-rbcL_p5 TGGGCCAATAATTTGAGCGA TGGTGACATAAGTCCCTCCC 45.0 55.0 57.9 58.4 1081-1148 bp 1.0 82.4