Hotspot

trnT-GGU-psbD

LSC · 33369-34380 · 1012 bp

Back to Vanda

Pi 0.00879
MarkerSeek score 58.90
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1012
variable_sites
29
pi
0.00879
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.95
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1121-1173 bp 1.0 84.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1124-1176 bp 1.0 84.4
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1111-1163 bp 1.0 83.6
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG CCCTCCGTAACCAATCATCC 55.0 55.0 58.1 57.7 1095-1147 bp 1.0 83.5
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1114-1166 bp 1.0 83.4