Hotspot

psbM-trnD-GUC

LSC · 30778-31930 · 1153 bp

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Pi 0.0149
MarkerSeek score 57.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1153
variable_sites
58
pi
0.0149
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 CGCAGTAGCAATAAACGCAA CCATGCATATGGCTGGGATT 45.0 50.0 57.5 58.1 1254-1277 bp 1.0 81.6
psbM-trnD-GUC_p2 CGCAGTAGCAATAAACGCAA AGTTCAATCGGTCAGAGCAC 45.0 50.0 57.5 57.9 1232-1255 bp 1.0 81.6
psbM-trnD-GUC_p3 CGCAGTAGCAATAAACGCAA ATGCATATGGCTGGGATTGT 45.0 45.0 57.5 57.3 1252-1275 bp 1.0 76.7
psbM-trnD-GUC_p4 GCGCAGTAGCAATAAACGC CCATGCATATGGCTGGGATT 52.6 50.0 58.2 58.1 1255-1278 bp 1.0 76.5
psbM-trnD-GUC_p5 GCGCAGTAGCAATAAACGC AGTTCAATCGGTCAGAGCAC 52.6 50.0 58.2 57.9 1233-1256 bp 1.0 76.5