Hotspot

rps3

LSC · 85732-86397 · 666 bp

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Pi 0.178

MarkerSeek score 80.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 666
variable_sites: 117
pi: 0.178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TTCCTGAGCCAATCTCCTCA	AATTGATCAGATTCGGGGGC	50.0	50.0	58.0	58.0	1803-1934 bp	1.0	90.8
rps3_p2	TTCCTGAGCCAATCTCCTCA	TAGTCAAGCCGAAGTGAACG	50.0	50.0	58.0	57.9	1650-1781 bp	1.0	90.8
rps3_p3	TTCCTGAGCCAATCTCCTCA	CGCACCTACTCTGACTTTCC	50.0	55.0	58.0	58.0	1994-2125 bp	1.0	90.6
rps3_p4	TTCCTGAGCCAATCTCCTCA	GCACCTACTCTGACTTTCCG	50.0	55.0	58.0	58.0	1993-2124 bp	1.0	90.6
rps3_p5	TTCCTGAGCCAATCTCCTCA	TCCCGGGCATCTACCATTAT	50.0	50.0	58.0	58.0	2113-2244 bp	1.0	90.2