Hotspot

rpl32

SSC · 117633-117755 · 123 bp

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Pi 0.0163

MarkerSeek score 61.80

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 123
variable_sites: 2
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	AGAAGTCTTACCCAGTTAACT	GAGCGATTCGTTTGCATTCA	38.1	45.0	53.5	57.5	244-245 bp	1.0	40.7
rpl32_p2	AGAAGTCTTACCCAGTTAACT	TGAGCGATTCGTTTGCATTC	38.1	45.0	53.5	57.5	245-246 bp	1.0	40.7
rpl32_p3	TGATCAATAGAAGTCTTACCCAGT	GAGCGATTCGTTTGCATTCA	37.5	45.0	56.9	57.5	252-253 bp	1.0	40.6
rpl32_p4	TGATCAATAGAAGTCTTACCCAGT	TGAGCGATTCGTTTGCATTC	37.5	45.0	56.9	57.5	253-254 bp	1.0	40.6
rpl32_p5	TGATCAATAGAAGTCTTACCCAGTT	GAGCGATTCGTTTGCATTCA	36.0	45.0	57.5	57.5	252-253 bp	1.0	40.6