Hotspot

rpl16

LSC · 83964-85532 · 1569 bp

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Pi 0.133

MarkerSeek score 80.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1569
variable_sites: 207
pi: 0.133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GTGGGGAACTGCTTCTTTGA	AAAAGGCATCGACCTGATCC	50.0	50.0	58.0	58.0	2506-2637 bp	1.0	89.9
rpl16_p2	CGCACCAAAAACTCGAGTTC	AAAAGGCATCGACCTGATCC	50.0	50.0	58.0	58.0	2662-2793 bp	1.0	89.8
rpl16_p3	CGCACCAAAAACTCGAGTTC	CGCAGGACGTATTGAAGGAA	50.0	50.0	58.0	58.0	2359 bp	0.5	59.8
rpl16_p4	GTGGGGAACTGCTTCTTTGA	CGCAGGACGTATTGAAGGAA	50.0	50.0	58.0	58.0	2203 bp	0.5	59.8
rpl16_p5	CGCACCAAAAACTCGAGTTC	GCAAACCATTCGAGCGAAAA	50.0	45.0	58.0	58.0	2293 bp	0.5	59.5