Hotspot

clpP

LSC · 72498-74661 · 2164 bp

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Pi 0.0065

MarkerSeek score 47.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2164
variable_sites: 14
pi: 0.0065
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGTGCTGCGAAATAGAAGGA	CCCATGAAAATACCCCCTCG	50.0	55.0	58.0	58.0	2718-2844 bp	1.0	83.8
clpP_p2	AGGTGCTGCGAAATAGAAGG	CCCATGAAAATACCCCCTCG	50.0	55.0	58.0	58.0	2719-2845 bp	1.0	83.8
clpP_p3	GTTTCCGCTGCAGATCCTTA	CCCATGAAAATACCCCCTCG	50.0	55.0	58.0	58.0	2609-2615 bp	1.0	80.4
clpP_p4	GTTTCCGCTGCAGATCCTTA	CCCCTGTGATATTCCAACCG	50.0	55.0	58.0	58.0	2851-2857 bp	1.0	80.3
clpP_p5	GTTTCCGCTGCAGATCCTTA	CTTCCGCCTTTCAGTTCAGT	50.0	50.0	58.0	58.1	2535-2541 bp	1.0	79.7