Hotspot

ndhD-psaC

SSC · 121389-121523 · 135 bp

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Pi 0.0746

MarkerSeek score 85.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 135
variable_sites: 10
pi: 0.0746
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ATAAAATGAGAGAGCCCGCG	TATGGCATGAGACAACTCGC	50.0	50.0	58.1	58.1	243 bp	1.0	82.1
ndhD-psaC_p2	CGCGAATATCGGCAAAACAA	TATGGCATGAGACAACTCGC	45.0	50.0	57.8	58.1	227 bp	1.0	82.1
ndhD-psaC_p3	ATAAAATGAGAGAGCCCGCG	CGGATTTCCTGAGTGTACGG	50.0	55.0	58.1	58.1	270 bp	1.0	81.6
ndhD-psaC_p4	CGCGAATATCGGCAAAACAA	CGGATTTCCTGAGTGTACGG	45.0	55.0	57.8	58.1	254 bp	1.0	81.5
ndhD-psaC_p5	ATAAAATGAGAGAGCCCGCG	GAAGACTGCGTGGGTTGTAA	50.0	50.0	58.1	58.1	313 bp	1.0	80.8