Hotspot

rpl16-rps3

LSC · 85533-85731 · 199 bp

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Pi 0.493

MarkerSeek score 71.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 73
pi: 0.493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TTCCTGAGCCAATCTCCTCA	AAAAGGCATCGACCTGATCC	50.0	50.0	58.0	58.0	1192-1323 bp	1.0	91.3
rpl16-rps3_p2	TTCCTGAGCCAATCTCCTCA	CAAGCCAAAGCCATCATTCG	50.0	50.0	58.0	58.1	1349-1480 bp	1.0	91.0
rpl16-rps3_p3	TTCCTGAGCCAATCTCCTCA	CGCAGGACGTATTGAAGGAA	50.0	50.0	58.0	58.0	889 bp	0.5	59.6
rpl16-rps3_p4	TTCCTGAGCCAATCTCCTCA	GCAAACCATTCGAGCGAAAA	50.0	45.0	58.0	58.0	823 bp	0.5	59.3
rpl16-rps3_p5	TTCCTGAGCCAATCTCCTCA	GGAAACGAAATTGCACGTGT	50.0	45.0	58.0	57.9	873 bp	0.5	58.7