Hotspot

accD-psaI

LSC · 61567-62463 · 897 bp

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Pi 0.0161

MarkerSeek score 71.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 14
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAGGCGTTCTGAGTGAGTTG	ACTAGGCCCACGATAGGTAC	50.0	55.0	58.1	58.0	1003-1033 bp	1.0	82.4
accD-psaI_p2	AAGGCGTTCTGAGTGAGTTG	CTAGGCCCACGATAGGTACA	50.0	55.0	58.1	58.0	1002-1032 bp	1.0	82.4
accD-psaI_p3	AAGGCGTTCTGAGTGAGTTG	GCAGGAAATACTAGGCCCAC	50.0	55.0	58.1	58.0	1012-1042 bp	1.0	82.1
accD-psaI_p4	AAGGCGTTCTGAGTGAGTTG	AGGAAATACTAGGCCCACGA	50.0	50.0	58.1	57.8	1010-1040 bp	1.0	81.0
accD-psaI_p5	AAGGCGTTCTGAGTGAGTTG	CAGGAAATACTAGGCCCACG	50.0	55.0	58.1	57.8	1011-1041 bp	1.0	80.5