Pi
0.00232
MarkerSeek score
60.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CTCTAACCGCTGAGCTAAGC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 906 bp | 1.0 | 78.9 |
| trnT-UGU-trnL-UAA_p2 | CTCTAACCGCTGAGCTAAGC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 914 bp | 1.0 | 78.4 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 944 bp | 1.0 | 78.2 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 952 bp | 1.0 | 77.7 |
| trnT-UGU-trnL-UAA_p5 | CAAATGCGATGCTCTAACCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.6 | 58.0 | 917 bp | 1.0 | 77.1 |