Hotspot

rpl14-rps3

LSC · 86754-87495 · 742 bp

Back to Vallisneria

Pi 0.128

MarkerSeek score 80.80

Primer available yes

Label rpl14-rps3

Feature Metadata

label_name: rpl14-rps3
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 93
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps3_p1	AGCAATTGCACCAAAAACCC	TGGTTCGCAGAACCAAAAGA	45.0	45.0	58.0	58.0	1645 bp	0.5	59.4
rpl14-rps3_p2	AGCAATTGCACCAAAAACCC	CCACTAGGTTTCCGACTTGG	45.0	55.0	58.0	57.9	1690 bp	0.5	59.0
rpl14-rps3_p3	CGAGACCCCCTTGGATTTTT	TGGTTCGCAGAACCAAAAGA	50.0	45.0	57.8	58.0	1626 bp	0.5	57.9
rpl14-rps3_p4	TGTCGCCGTAGAGTTGAAAC	TGGTTCGCAGAACCAAAAGA	50.0	45.0	58.2	58.0	2055 bp	0.5	57.8
rpl14-rps3_p5	CAATTGCACCAAAAACCCGA	TGGTTCGCAGAACCAAAAGA	45.0	45.0	57.8	58.0	1643 bp	0.5	57.8