Hotspot

rps18-rps12

LSC · 72942-74702 · 1761 bp

Back to Vallisneria

Pi 0.175

MarkerSeek score 80.40

Primer available yes

Label rps18-rps12

Feature Metadata

label_name: rps18-rps12
feature_type: igs
region: LSC
length_bp: 1761
variable_sites: 264
pi: 0.175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rps12_p1	TAGACGTTTGCCCGCAATTA	CCTGTACGGCTGTTCTCTTC	45.0	55.0	57.9	58.0	2672 bp	0.5	59.2
rps18-rps12_p2	TAGACGTTTGCCCGCAATTA	TATATCAGCAGACGAGGCCA	45.0	50.0	57.9	58.0	2413 bp	0.5	59.2
rps18-rps12_p3	TAGACGTTTGCCCGCAATTA	AGACGAGGCCAAAGCTTATG	45.0	50.0	57.9	58.0	2404 bp	0.5	59.0
rps18-rps12_p4	TAGACGTTTGCCCGCAATTA	CAGACGAGGCCAAAGCTTAT	45.0	50.0	57.9	58.0	2405 bp	0.5	59.0
rps18-rps12_p5	TAGACGTTTGCCCGCAATTA	TGAGCATTTTGTGTCACGGA	45.0	45.0	57.9	58.0	2561 bp	0.5	58.9