Hotspot

psbE-petG

LSC · 69441-70761 · 1321 bp

Back to Vallisneria

Pi 0.175

MarkerSeek score 83.60

Primer available yes

Label psbE-petG

Feature Metadata

label_name: psbE-petG
feature_type: igs
region: LSC
length_bp: 1321
variable_sites: 201
pi: 0.175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petG_p1	AAAAGAGCGTTCTCCCGTG	TGATCACCACGCCTGTATTG	52.6	50.0	58.1	58.0	1383-1452 bp	1.0	83.1
psbE-petG_p2	CAAAAGAGCGTTCTCCCGT	TGATCACCACGCCTGTATTG	52.6	50.0	58.1	58.0	1384-1453 bp	1.0	83.1
psbE-petG_p3	AAAAGAGCGTTCTCCCGTG	GATCACCACGCCTGTATTGT	52.6	50.0	58.1	58.0	1382-1451 bp	1.0	83.1
psbE-petG_p4	CAAAAGAGCGTTCTCCCGT	GATCACCACGCCTGTATTGT	52.6	50.0	58.1	58.0	1383-1452 bp	1.0	83.1
psbE-petG_p5	AAAAGAGCGTTCTCCCGTG	CTGATCACCACGCCTGTATT	52.6	50.0	58.1	57.7	1384-1453 bp	1.0	80.8