Hotspot

ycf3-trnF-GAA

LSC · 52558-53128 · 571 bp

Back to Vallisneria

Pi 0.469

MarkerSeek score 82.50

Primer available yes

Label ycf3-trnF-GAA

Feature Metadata

label_name: ycf3-trnF-GAA
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 250
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnF-GAA_p1	GAATGGATTGCTGAGCCGTA	CCGTCGACTTGAGAAATCGT	50.0	50.0	58.0	58.0	1301 bp	0.5	59.5
ycf3-trnF-GAA_p2	CAGAATTTTGGAAACGCGCT	CCGTCGACTTGAGAAATCGT	45.0	50.0	57.9	58.0	1158 bp	0.5	59.4
ycf3-trnF-GAA_p3	AGGGCACAGAATTGGTTGAG	CCGTCGACTTGAGAAATCGT	50.0	50.0	58.1	58.0	1111 bp	0.5	59.1
ycf3-trnF-GAA_p4	GGGCACAGAATTGGTTGAGA	CCGTCGACTTGAGAAATCGT	50.0	50.0	58.1	58.0	1110 bp	0.5	59.1
ycf3-trnF-GAA_p5	GTTTGGTTCGATCATGCTGC	CCGTCGACTTGAGAAATCGT	50.0	50.0	58.1	58.0	1177 bp	0.5	59.0