Hotspot

trnS-GGA-ycf3

LSC · 49707-50494 · 788 bp

Back to Vallisneria

Pi 0.469

MarkerSeek score 79.70

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 300
pi: 0.469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	CTACACCTTCAACCACTCGG	ATGCTTGTTGTCCCAACCAT	55.0	45.0	57.9	58.0	1330 bp	0.5	59.2
trnS-GGA-ycf3_p2	GATCGGTCAGATTGGCGAAT	ATGCTTGTTGTCCCAACCAT	50.0	45.0	58.1	58.0	1429 bp	0.5	58.9
trnS-GGA-ycf3_p3	TGGCGAATTCGATGGATGAG	ATGCTTGTTGTCCCAACCAT	50.0	45.0	58.1	58.0	1417 bp	0.5	58.8
trnS-GGA-ycf3_p4	GGCGAATTCGATGGATGAGA	ATGCTTGTTGTCCCAACCAT	50.0	45.0	57.8	58.0	1416 bp	0.5	58.6
trnS-GGA-ycf3_p5	CTACACCTTCAACCACTCGG	TCAACGCCTCTGATTTCCAG	55.0	50.0	57.9	57.9	1415 bp	0.5	58.6