Hotspot

trnT-UGU-rps4

LSC · 47948-48441 · 494 bp

Back to Vallisneria

Pi 0.447

MarkerSeek score 81.50

Primer available yes

Label trnT-UGU-rps4

Feature Metadata

label_name: trnT-UGU-rps4
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 209
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-rps4_p1	GAGAAACCCCTTTTCCTGCA	ACTAATTGCCTGGCGTTAGG	50.0	50.0	58.0	58.0	1206 bp	0.5	59.7
trnT-UGU-rps4_p2	CCGCCAATAGCCAAGTTTTG	ACTAATTGCCTGGCGTTAGG	50.0	50.0	58.0	58.0	1416 bp	0.5	59.7
trnT-UGU-rps4_p3	TGAACTCAAATGCCTGTGCT	ACTAATTGCCTGGCGTTAGG	45.0	50.0	58.0	58.0	943 bp	0.5	59.6
trnT-UGU-rps4_p4	GTGAACTCAAATGCCTGTGC	ACTAATTGCCTGGCGTTAGG	50.0	50.0	57.9	58.0	944 bp	0.5	59.2
trnT-UGU-rps4_p5	GAGAAACCCCTTTTCCTGCA	CCTGGCGTTAGGAATTGTCA	50.0	50.0	58.0	57.9	1198 bp	0.5	59.1