primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-rps14_p1	trnM-CAU-rps14	1	GAACCCGTGACTTCAAGGTT	GCGCAACAAGATCAAGTTGG	20	20	50.0	50.0	58.0	58.3	12.6	23.3	0.346869	42197	42334	218-247	1.000000	86.5
trnM-CAU-rps14_p2	trnM-CAU-rps14	2	GAACCCGTGACTTCAAGGTT	GGCGCAACAAGATCAAGTTG	20	20	50.0	50.0	58.0	58.3	12.6	19.9	0.346869	42197	42334	219-248	1.000000	86.5
trnM-CAU-rps14_p3	trnM-CAU-rps14	3	TTGAACCCGTGACTTCAAGG	GCGCAACAAGATCAAGTTGG	20	20	50.0	50.0	58.0	58.3	4.3	23.3	0.348247	42197	42334	220-249	1.000000	86.4
trnM-CAU-rps14_p4	trnM-CAU-rps14	4	TTGAACCCGTGACTTCAAGG	GGCGCAACAAGATCAAGTTG	20	20	50.0	50.0	58.0	58.3	4.3	19.9	0.348247	42197	42334	221-250	1.000000	86.4
trnM-CAU-rps14_p5	trnM-CAU-rps14	5	GGGATTTGAACCCGTGACTT	GCGCAACAAGATCAAGTTGG	20	20	50.0	50.0	58.1	58.3	0.0	23.3	0.404403	42197	42334	225-254	1.000000	85.8
trnT-UGU-rps4_p1	trnT-UGU-rps4	1	GAGAAACCCCTTTTCCTGCA	ACTAATTGCCTGGCGTTAGG	20	20	50.0	50.0	58.0	58.0	0.0	30.5	0.043011	47948	48441	1206-1206	0.500000	59.7
trnT-UGU-rps4_p2	trnT-UGU-rps4	2	CCGCCAATAGCCAAGTTTTG	ACTAATTGCCTGGCGTTAGG	20	20	50.0	50.0	58.0	58.0	0.0	30.5	0.043471	47948	48441	1416-1416	0.500000	59.7
trnT-UGU-rps4_p3	trnT-UGU-rps4	3	TGAACTCAAATGCCTGTGCT	ACTAATTGCCTGGCGTTAGG	20	20	45.0	50.0	58.0	58.0	0.0	30.5	0.052914	47948	48441	943-943	0.500000	59.6
trnT-UGU-rps4_p4	trnT-UGU-rps4	4	GTGAACTCAAATGCCTGTGC	ACTAATTGCCTGGCGTTAGG	20	20	50.0	50.0	57.9	58.0	0.0	30.5	0.094481	47948	48441	944-944	0.500000	59.2
trnT-UGU-rps4_p5	trnT-UGU-rps4	5	GAGAAACCCCTTTTCCTGCA	CCTGGCGTTAGGAATTGTCA	20	20	50.0	50.0	58.0	57.9	0.0	1.3	0.112574	47948	48441	1198-1198	0.500000	59.1
trnS-GGA-ycf3_p1	trnS-GGA-ycf3	1	CTACACCTTCAACCACTCGG	ATGCTTGTTGTCCCAACCAT	20	20	55.0	45.0	57.9	58.0	0.0	4.8	0.094296	49707	50494	1330-1330	0.500000	59.2
trnS-GGA-ycf3_p2	trnS-GGA-ycf3	2	GATCGGTCAGATTGGCGAAT	ATGCTTGTTGTCCCAACCAT	20	20	50.0	45.0	58.1	58.0	0.0	4.8	0.142930	49707	50494	1429-1429	0.500000	58.9
trnS-GGA-ycf3_p3	trnS-GGA-ycf3	3	TGGCGAATTCGATGGATGAG	ATGCTTGTTGTCCCAACCAT	20	20	50.0	45.0	58.1	58.0	31.0	4.8	0.144236	49707	50494	1417-1417	0.500000	58.8
trnS-GGA-ycf3_p4	trnS-GGA-ycf3	4	GGCGAATTCGATGGATGAGA	ATGCTTGTTGTCCCAACCAT	20	20	50.0	45.0	57.8	58.0	31.0	4.8	0.168863	49707	50494	1416-1416	0.500000	58.6
trnS-GGA-ycf3_p5	trnS-GGA-ycf3	5	CTACACCTTCAACCACTCGG	TCAACGCCTCTGATTTCCAG	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.179427	49707	50494	1415-1415	0.500000	58.6
ycf3_p1	ycf3	1	AGGAAGGGGGATCTTCATGT	ATCCTCTCCTTCCGGGTTTT	20	20	50.0	50.0	57.7	58.3	6.9	0.0	0.623539	50495	52557	2949-2949	0.500000	55.0
ycf3_p2	ycf3	2	ATGTTTGTCAAGACCGACGG	ATCCTCTCCTTCCGGGTTTT	20	20	50.0	50.0	58.5	58.3	13.6	0.0	0.821309	50495	52557	2933-2933	0.500000	53.4
ycf3_p3	ycf3	3	AGGAAGGGGGATCTTCATGT	TATCCTCTCCTTCCGGGTTT	20	20	50.0	50.0	57.7	57.4	6.9	0.0	0.856637	50495	52557	2950-2950	0.500000	53.1
ycf3_p4	ycf3	4	AGGAAGGGGGATCTTCATGT	TTATCCTCTCCTTCCGGGTT	20	20	50.0	50.0	57.7	57.4	6.9	0.0	0.856637	50495	52557	2951-2951	0.500000	53.1
ycf3_p5	ycf3	5	AGGAAGGGGGATCTTCATGT	TTTATCCTCTCCTTCCGGGT	20	20	50.0	50.0	57.7	57.4	6.9	0.0	0.856637	50495	52557	2952-2952	0.500000	53.1
ycf3-trnF-GAA_p1	ycf3-trnF-GAA	1	GAATGGATTGCTGAGCCGTA	CCGTCGACTTGAGAAATCGT	20	20	50.0	50.0	58.0	58.0	0.2	14.7	0.067899	52558	53128	1301-1301	0.500000	59.5
ycf3-trnF-GAA_p2	ycf3-trnF-GAA	2	CAGAATTTTGGAAACGCGCT	CCGTCGACTTGAGAAATCGT	20	20	45.0	50.0	57.9	58.0	5.7	14.7	0.071935	52558	53128	1158-1158	0.500000	59.4
ycf3-trnF-GAA_p3	ycf3-trnF-GAA	3	AGGGCACAGAATTGGTTGAG	CCGTCGACTTGAGAAATCGT	20	20	50.0	50.0	58.1	58.0	0.0	14.7	0.106317	52558	53128	1111-1111	0.500000	59.1
ycf3-trnF-GAA_p4	ycf3-trnF-GAA	4	GGGCACAGAATTGGTTGAGA	CCGTCGACTTGAGAAATCGT	20	20	50.0	50.0	58.1	58.0	0.0	14.7	0.111758	52558	53128	1110-1110	0.500000	59.1
ycf3-trnF-GAA_p5	ycf3-trnF-GAA	5	GTTTGGTTCGATCATGCTGC	CCGTCGACTTGAGAAATCGT	20	20	50.0	50.0	58.1	58.0	0.0	14.7	0.121221	52558	53128	1177-1177	0.500000	59.0
trnL-UAA_p1	trnL-UAA	1	GGGACAGGGACTCAAAAAGG	TGAATCAGAGCACATGGAGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.123047	53582	54484	1589-1589	0.500000	59.0
trnL-UAA_p2	trnL-UAA	2	AAGAAAGGGACAGGGACTCA	TGAATCAGAGCACATGGAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.152508	53582	54484	1595-1595	0.500000	58.8
trnL-UAA_p3	trnL-UAA	3	AGAAAGGGACAGGGACTCAA	TGAATCAGAGCACATGGAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.152508	53582	54484	1594-1594	0.500000	58.8
trnL-UAA_p4	trnL-UAA	4	CATGCCACCAAAAACTTGGG	TGAATCAGAGCACATGGAGC	20	20	50.0	50.0	58.1	58.0	8.7	0.0	0.152514	53582	54484	1415-1415	0.500000	58.8
trnL-UAA_p5	trnL-UAA	5	GAGCTATCCTGACTCAACGC	TGAATCAGAGCACATGGAGC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.187563	53582	54484	1654-1654	0.500000	58.5
trnL-UAA-trnM-CAU_p1	trnL-UAA-trnM-CAU	1	ACCAATTTCGCCATATCCCC	AGCGAATTTGAGTAGAGCGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.076729	54485	55554	1559-1559	0.500000	59.4
trnL-UAA-trnM-CAU_p2	trnL-UAA-trnM-CAU	2	ACCAATTTCGCCATATCCCC	ACGACCGATGGTTAACAGTG	20	20	50.0	50.0	58.0	57.9	0.0	8.1	0.079171	54485	55554	1698-1698	0.500000	59.4
trnL-UAA-trnM-CAU_p3	trnL-UAA-trnM-CAU	3	ACCAATTTCGCCATATCCCC	GCGAATTTGAGTAGAGCGGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.082053	54485	55554	1558-1558	0.500000	59.3
trnL-UAA-trnM-CAU_p4	trnL-UAA-trnM-CAU	4	TCAGGATTGCCCGTTTTTCA	AGCGAATTTGAGTAGAGCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.083812	54485	55554	1661-1661	0.500000	59.3
trnL-UAA-trnM-CAU_p5	trnL-UAA-trnM-CAU	5	TCAGGATTGCCCGTTTTTCA	ACGACCGATGGTTAACAGTG	20	20	45.0	50.0	58.0	57.9	0.0	8.1	0.086254	54485	55554	1800-1800	0.500000	59.3
psbE-petG_p1	psbE-petG	1	AAAAGAGCGTTCTCCCGTG	TGATCACCACGCCTGTATTG	19	20	52.6	50.0	58.1	58.0	1.0	0.0	1.108385	69441	70761	1383-1452	1.000000	83.1
psbE-petG_p2	psbE-petG	2	CAAAAGAGCGTTCTCCCGT	TGATCACCACGCCTGTATTG	19	20	52.6	50.0	58.1	58.0	1.0	0.0	1.108385	69441	70761	1384-1453	1.000000	83.1
psbE-petG_p3	psbE-petG	3	AAAAGAGCGTTCTCCCGTG	GATCACCACGCCTGTATTGT	19	20	52.6	50.0	58.1	58.0	1.0	0.0	1.109796	69441	70761	1382-1451	1.000000	83.1
psbE-petG_p4	psbE-petG	4	CAAAAGAGCGTTCTCCCGT	GATCACCACGCCTGTATTGT	19	20	52.6	50.0	58.1	58.0	1.0	0.0	1.109796	69441	70761	1383-1452	1.000000	83.1
psbE-petG_p5	psbE-petG	5	AAAAGAGCGTTCTCCCGTG	CTGATCACCACGCCTGTATT	19	20	52.6	50.0	58.1	57.7	1.0	0.0	1.401546	69441	70761	1384-1453	1.000000	80.8
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCGTGTTCT	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.165195	71051	71262	309-396	1.000000	90.5
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCGTGTTCT	CCGGTAGCGTGTTTGTTTTG	20	20	50.0	50.0	58.0	58.3	0.0	14.0	0.287675	71051	71262	311-398	1.000000	89.5
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCGTGTTCT	CGGTAGCGTGTTTGTTTTGG	20	20	50.0	50.0	58.0	58.3	0.0	6.1	0.287675	71051	71262	310-397	1.000000	89.5
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	ACCCGTGTTCTACCAAACTG	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.378211	71051	71262	300-387	1.000000	88.9
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	ATTTGAACCTACGGCATCGG	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.464673	71051	71262	329-416	1.000000	87.9
trnP-UGG-rps18_p1	trnP-UGG-rps18	1	CAATACAGGCGTGGTGATCA	CGAGTATTTCTAGGACCGCG	20	20	50.0	55.0	58.0	58.0	11.7	0.0	0.056558	71337	72797	2210-2210	0.500000	59.5
trnP-UGG-rps18_p2	trnP-UGG-rps18	2	ACAATACAGGCGTGGTGATC	CGAGTATTTCTAGGACCGCG	20	20	50.0	55.0	58.0	58.0	11.7	0.0	0.057969	71337	72797	2211-2211	0.500000	59.5
trnP-UGG-rps18_p3	trnP-UGG-rps18	3	CAATACAGGCGTGGTGATCA	AGTCCAAGAAGAAGGAGGCT	20	20	50.0	50.0	58.0	58.0	11.7	0.0	0.060150	71337	72797	2313-2313	0.500000	59.5
trnP-UGG-rps18_p4	trnP-UGG-rps18	4	ACAATACAGGCGTGGTGATC	AGTCCAAGAAGAAGGAGGCT	20	20	50.0	50.0	58.0	58.0	11.7	0.0	0.061561	71337	72797	2314-2314	0.500000	59.5
trnP-UGG-rps18_p5	trnP-UGG-rps18	5	GTTTTGGAGACCCGTGTTCT	CGAGTATTTCTAGGACCGCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.070621	71337	72797	2043-2043	0.500000	59.4
rps18-rps12_p1	rps18-rps12	1	TAGACGTTTGCCCGCAATTA	CCTGTACGGCTGTTCTCTTC	20	20	45.0	55.0	57.9	58.0	2.4	2.0	0.098551	72942	74702	2672-2672	0.500000	59.2
rps18-rps12_p2	rps18-rps12	2	TAGACGTTTGCCCGCAATTA	TATATCAGCAGACGAGGCCA	20	20	45.0	50.0	57.9	58.0	2.4	5.0	0.100844	72942	74702	2413-2413	0.500000	59.2
rps18-rps12_p3	rps18-rps12	3	TAGACGTTTGCCCGCAATTA	AGACGAGGCCAAAGCTTATG	20	20	45.0	50.0	57.9	58.0	2.4	5.0	0.126058	72942	74702	2404-2404	0.500000	59.0
rps18-rps12_p4	rps18-rps12	4	TAGACGTTTGCCCGCAATTA	CAGACGAGGCCAAAGCTTAT	20	20	45.0	50.0	57.9	58.0	2.4	5.0	0.126058	72942	74702	2405-2405	0.500000	59.0
rps18-rps12_p5	rps18-rps12	5	TAGACGTTTGCCCGCAATTA	TGAGCATTTTGTGTCACGGA	20	20	45.0	45.0	57.9	58.0	2.4	0.0	0.135803	72942	74702	2561-2561	0.500000	58.9
rpl14-rps3_p1	rpl14-rps3	1	AGCAATTGCACCAAAAACCC	TGGTTCGCAGAACCAAAAGA	20	20	45.0	45.0	58.0	58.0	30.9	28.8	0.079627	86754	87495	1645-1645	0.500000	59.4
rpl14-rps3_p2	rpl14-rps3	2	AGCAATTGCACCAAAAACCC	CCACTAGGTTTCCGACTTGG	20	20	45.0	55.0	58.0	57.9	30.9	0.0	0.128828	86754	87495	1690-1690	0.500000	59.0
rpl14-rps3_p3	rpl14-rps3	3	CGAGACCCCCTTGGATTTTT	TGGTTCGCAGAACCAAAAGA	20	20	50.0	45.0	57.8	58.0	0.0	28.8	0.261503	86754	87495	1626-1626	0.500000	57.9
rpl14-rps3_p4	rpl14-rps3	4	TGTCGCCGTAGAGTTGAAAC	TGGTTCGCAGAACCAAAAGA	20	20	50.0	45.0	58.2	58.0	0.0	28.8	0.270923	86754	87495	2055-2055	0.500000	57.8
rpl14-rps3_p5	rpl14-rps3	5	CAATTGCACCAAAAACCCGA	TGGTTCGCAGAACCAAAAGA	20	20	45.0	45.0	57.8	58.0	0.0	28.8	0.276776	86754	87495	1643-1643	0.500000	57.8
rps3_p1	rps3	1	CGTGCATATCGTGTCATTGC	TGTCTGTTTTCAAAGCCCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.054791	87496	88152	1429-1429	0.500000	59.6
rps3_p2	rps3	2	TCGCCCAGCTTCTATTTGTC	TGTCTGTTTTCAAAGCCCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.069737	87496	88152	1406-1406	0.500000	59.4
rps3_p3	rps3	3	CGCCCAGCTTCTATTTGTCT	TGTCTGTTTTCAAAGCCCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.075400	87496	88152	1405-1405	0.500000	59.4
rps3_p4	rps3	4	CGTGCATATCGTGTCATTGC	GATCAAATTCGCGGACGTTC	20	20	50.0	50.0	58.0	57.9	0.0	6.1	0.082219	87496	88152	1400-1400	0.500000	59.3
rps3_p5	rps3	5	TTTGCCGTTGCTCTTACTGT	TGTCTGTTTTCAAAGCCCGA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.083768	87496	88152	1495-1495	0.500000	59.3