Hotspot

ycf1

SSC · 119549-125020 · 5472 bp

Back to Valeriana

Pi 0.0704

MarkerSeek score 78.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 1111
pi: 0.0704
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAAGAGCACAGGCAATTTT	TGCACCAATTTACGTGGACA	45.0	45.0	57.8	58.0	471-660 bp	0.875	85.5
ycf1_p2	CGGGTTTTGTGGGTTTTAGC	TGCACCAATTTACGTGGACA	50.0	45.0	57.9	58.0	720-909 bp	0.75	79.4
ycf1_p3	GCGGGTTTTGTGGGTTTTAG	TGCACCAATTTACGTGGACA	50.0	45.0	57.9	58.0	721-910 bp	0.75	79.4
ycf1_p4	CCCTTGTCTTTTCGTCCACA	TGCACCAATTTACGTGGACA	50.0	45.0	58.0	58.0	477-513 bp	0.25	53.4
ycf1_p5	CCCTTGTCTTTTCGTCCACA	TCCTTTTTCCCAGTTCGACG	50.0	50.0	58.0	58.1	705-741 bp	0.25	52.7