Hotspot

accD

LSC · 55903-57639 · 1737 bp

Back to Valeriana

Pi 0.0646

MarkerSeek score 76.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1737
variable_sites: 282
pi: 0.0646
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGGGCTAGATGGAGGTCAAA	CCAACAGCGATTCTGAGTCA	50.0	50.0	58.0	57.9	1989-2274 bp	0.5	66.8
accD_p2	TTGGGCTAGATGGAGGTCAA	CCAACAGCGATTCTGAGTCA	50.0	50.0	58.0	57.9	1990-2275 bp	0.5	66.8
accD_p3	TTTGGGCTAGATGGAGGTCA	CCAACAGCGATTCTGAGTCA	50.0	50.0	58.0	57.9	1991-2276 bp	0.5	66.8
accD_p4	TGGGCTAGATGGAGGTCAAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	2396-2690 bp	0.5	66.3
accD_p5	TTGGGCTAGATGGAGGTCAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	2397-2691 bp	0.5	66.3