Hotspot

ndhG-ndhI

SSC · 114713-115077 · 365 bp

Back to Valeriana

Pi 0.0576

MarkerSeek score 70.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 365
variable_sites: 59
pi: 0.0576
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AACACCACTCCTAGACCTCC	GCTGCGAGATACATTGGTCA	55.0	50.0	58.1	58.1	890-901 bp	1.0	88.6
ndhG-ndhI_p2	AACACCACTCCTAGACCTCC	TGTGAAGTATGTGTTCGCGT	55.0	45.0	58.1	57.9	743-754 bp	1.0	88.3
ndhG-ndhI_p3	TGGGAAGTAACACCACTCCT	GCTGCGAGATACATTGGTCA	50.0	50.0	57.9	58.1	898-909 bp	1.0	88.2
ndhG-ndhI_p4	CGCGTAGAGTTGAAGGGAAA	GCTGCGAGATACATTGGTCA	50.0	50.0	57.9	58.1	1425-1436 bp	1.0	87.5
ndhG-ndhI_p5	TCCCAACGAAAAGGCAGAAA	GCTGCGAGATACATTGGTCA	45.0	50.0	57.9	58.1	927-938 bp	0.875	80.1