Hotspot

rpl22-rps19

LSC · 83135-83225 · 91 bp

Back to Valeriana

Pi 0.0651

MarkerSeek score 68.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 16
pi: 0.0651
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGGAACGTCCACGAATT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	747-759 bp	1.0	85.8
rpl22-rps19_p2	TTGTCCCATAGTCCCCCATT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1108-1120 bp	1.0	85.2
rpl22-rps19_p3	TTTGTCCCATAGTCCCCCAT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1109-1121 bp	1.0	85.2
rpl22-rps19_p4	TTGTCCCATAGTCCCCCATT	GAGGAGTAAGCTGTGACACG	50.0	55.0	58.0	58.0	845-856 bp	0.875	77.1
rpl22-rps19_p5	TTTGTCCCATAGTCCCCCAT	GAGGAGTAAGCTGTGACACG	50.0	55.0	58.0	58.0	846-857 bp	0.875	77.1