Hotspot

accD-psaI

LSC · 57640-58219 · 580 bp

Back to Valeriana

Pi 0.0375

MarkerSeek score 69.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 580
variable_sites: 66
pi: 0.0375
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGCCTATATTGCATTTGCGG	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1363-1430 bp	0.875	84.2
accD-psaI_p2	GGTGACAGCCAGTTTTGGTA	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1414-1481 bp	0.875	84.1
accD-psaI_p3	TACTACTGGTGGGGTGACAG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.1	58.0	1426-1493 bp	0.875	83.6
accD-psaI_p4	AGTATGGGATCCGTAGTGGG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.0	58.0	1635-1702 bp	0.875	83.1
accD-psaI_p5	TCCTGTAGCAATGGGGGTTA	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	1678-1745 bp	0.875	82.8