Hotspot

rbcL-accD

LSC · 55199-55902 · 704 bp

Back to Valeriana

Pi 0.0618

MarkerSeek score 74.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 130
pi: 0.0618
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGAATGCTACTGCGGGTAC	AAACCAGACAAACTCGTGCT	50.0	45.0	58.0	58.0	1548-1567 bp	0.625	69.0
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	AACCAGACAAACTCGTGCTT	50.0	45.0	58.0	58.0	1547-1566 bp	0.625	68.9
rbcL-accD_p3	ATGAAATTATCCGTGCGGCT	TCTTTGTGCGAAACCAGACA	45.0	45.0	58.0	58.0	951-970 bp	0.375	58.7
rbcL-accD_p4	AATGAGGGACGTGATCTTGC	TCTTTGTGCGAAACCAGACA	50.0	45.0	58.0	58.0	982-1001 bp	0.375	58.6
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	TCTTTGTGCGAAACCAGACA	50.0	45.0	58.0	58.0	1558-1577 bp	0.375	57.3