Hotspot

ndhC-trnV-UAC

LSC · 49227-49949 · 723 bp

Back to Valeriana

Pi 0.0435

MarkerSeek score 68.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 91
pi: 0.0435
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAATGAAGCAACAGCTGGT	AGCCTGACAAATGGTTTGGT	45.0	45.0	58.0	57.9	1789-1988 bp	1.0	86.3
ndhC-trnV-UAC_p2	AAGGGCAACTCAACGGAATT	AGCCTGACAAATGGTTTGGT	45.0	45.0	58.0	57.9	1672-1881 bp	1.0	86.2
ndhC-trnV-UAC_p3	CTAAGACCATTCCAAGGCCC	AGCCTGACAAATGGTTTGGT	55.0	45.0	57.9	57.9	1601-1810 bp	1.0	85.9
ndhC-trnV-UAC_p4	AAGGGCAACTCAACGGAATT	ATGGTTTGGTCCGAATCAGG	45.0	50.0	58.0	57.9	1662-1861 bp	0.75	74.8
ndhC-trnV-UAC_p5	TCAATGAAGCAACAGCTGGT	ATGGTTTGGTCCGAATCAGG	45.0	50.0	58.0	57.9	1779-1978 bp	0.75	74.6