Hotspot

trnK-UUU-rps16

LSC · 3936-4499 · 564 bp

Back to Valeriana

Pi 0.0375

MarkerSeek score 69.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 66
pi: 0.0375
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTCAAAACTT	AAGGAGGGCAGAGGTTTTTG	45.0	50.0	58.1	58.0	741-788 bp	0.875	82.4
trnK-UUU-rps16_p2	ACTCGTGGAAAAACCCTTCC	AAGGAGGGCAGAGGTTTTTG	50.0	50.0	58.0	58.0	1044-1091 bp	0.875	81.3
trnK-UUU-rps16_p3	ACTCGTGGAAAAACCCTTCC	TGCCGAAGAGAAGGAAGAGA	50.0	50.0	58.0	58.1	1181-1228 bp	0.875	80.1
trnK-UUU-rps16_p4	ACTCGTGGAAAAACCCTTCC	CCACAGTGTATCACGCAAGA	50.0	50.0	58.0	57.9	1729 bp	0.25	49.2
trnK-UUU-rps16_p5	ACTCGTGGAAAAACCCTTCC	TCCACAGTGTATCACGCAAG	50.0	50.0	58.0	57.9	1730 bp	0.25	49.2