Hotspot

trnL-UAG-rpl32

SSC · 135363-135871 · 509 bp

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Pi 0.0319
MarkerSeek score 74.60
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
509
variable_sites
35
pi
0.0319
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC TCTCTTTCTACGGGGAACTCA 50.0 47.6 58.0 57.8 564-702 bp 1.0 79.1
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA TCTCTTTCTACGGGGAACTCA 50.0 47.6 58.0 57.8 565-703 bp 1.0 79.1
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AGCGAAATCTCTTTCTACGGG 50.0 47.6 58.0 57.8 571-709 bp 1.0 79.1
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA AGCGAAATCTCTTTCTACGGG 50.0 47.6 58.0 57.8 572-710 bp 1.0 79.0
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA TCTCTTTCTACGGGGAACTCA 50.0 47.6 57.9 57.8 574-712 bp 1.0 78.1