Hotspot

trnR-ACG-trnN-GUU

IRb · 118605-119220 · 616 bp

Back to Vachellia

Pi 0.00801
MarkerSeek score 65.20
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
616
variable_sites
10
pi
0.00801
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 ATAGATCCAGTGGAGACGGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 58.0 58.0 784-792 bp 1.0 82.3
trnR-ACG-trnN-GUU_p2 ATAGATCCAGTGGAGACGGG TGGGTTGAGAACGGGAATTG 55.0 50.0 58.0 58.1 825-833 bp 1.0 81.4
trnR-ACG-trnN-GUU_p3 ATAGATCCAGTGGAGACGGG GCTGTTAACTGACTGGTCGT 55.0 50.0 58.0 57.8 748-756 bp 1.0 81.2
trnR-ACG-trnN-GUU_p4 ATAGATCCAGTGGAGACGGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 58.0 58.2 735-743 bp 1.0 81.2
trnR-ACG-trnN-GUU_p5 ATAGATCCAGTGGAGACGGG GGGTTGAGAACGGGAATTGA 55.0 50.0 58.0 57.8 824-832 bp 1.0 80.6