Hotspot

rps8-rpl14

LSC · 89940-90497 · 558 bp

Back to Vachellia

Pi 0.0579

MarkerSeek score 72.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 42
pi: 0.0579
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GCAATGGTGTCCTTACCCAT	AGTTTCATTAGCACCCGAAGT	50.0	42.9	57.9	57.6	325-606 bp	1.0	79.0
rps8-rpl14_p2	TCAGCAATGGTGTCCTTACC	AGTTTCATTAGCACCCGAAGT	50.0	42.9	57.5	57.6	328-609 bp	1.0	76.2
rps8-rpl14_p3	CAGCAATGGTGTCCTTACCC	AGTTTCATTAGCACCCGAAGT	55.0	42.9	58.5	57.6	327-608 bp	1.0	75.9
rps8-rpl14_p4	ATGTCAGCAATGGTGTCCTT	AGTTTCATTAGCACCCGAAGT	45.0	42.9	57.4	57.6	331-612 bp	1.0	75.3
rps8-rpl14_p5	GCAATGGTGTCCTTACCCAT	GTTTCATTAGCACCCGAAGT	50.0	45.0	57.9	56.1	324-605 bp	1.0	74.8