Hotspot

rpl33-rps18

LSC · 76705-77104 · 400 bp

Back to Vachellia

Pi 0.0346

MarkerSeek score 76.30

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 26
pi: 0.0346
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	511-536 bp	1.0	84.7
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	512-537 bp	1.0	84.7
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	513-538 bp	1.0	82.9
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	485-510 bp	1.0	82.4
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	GGGCAAACGTCTACGAAAAG	45.0	50.0	57.9	57.4	486-511 bp	1.0	82.4