Hotspot

petA-psbJ

LSC · 71017-72387 · 1371 bp

Back to Vachellia

Pi 0.0182

MarkerSeek score 70.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1371
variable_sites: 53
pi: 0.0182
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTTCTTCGTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1492-1528 bp	1.0	80.5
petA-psbJ_p2	TTGTTCTTCGTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1493-1529 bp	1.0	80.5
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.4	57.6	1517-1553 bp	1.0	79.5
petA-psbJ_p4	GGTTTCTTGTTCTTCGTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	1499-1535 bp	1.0	79.3
petA-psbJ_p5	ACGTGTCCAAGGTTTCTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1509-1545 bp	1.0	79.2