Hotspot

ycf4-cemA

LSC · 68676-69137 · 462 bp

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Pi 0.0114

MarkerSeek score 69.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 12
pi: 0.0114
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGATCCACCAGGGCAAAAAG	45.0	50.0	58.1	58.1	552-583 bp	1.0	83.3
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	553-584 bp	1.0	83.2
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GATCCACCAGGGCAAAAAGA	45.0	50.0	58.1	58.1	551-582 bp	1.0	83.2
ycf4-cemA_p4	GAACAAAAAGCGGCGGAATT	AGATCCACCAGGGCAAAAAG	45.0	50.0	58.2	58.1	570-601 bp	1.0	82.1
ycf4-cemA_p5	GAACAAAAAGCGGCGGAATT	GATCCACCAGGGCAAAAAGA	45.0	50.0	58.2	58.1	569-600 bp	1.0	82.1