Hotspot

accD-psaI

LSC · 67001-67813 · 813 bp

Back to Vachellia

Pi 0.0458

MarkerSeek score 74.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 60
pi: 0.0458
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGCTTACATTGCATTTGCGG	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	952-1079 bp	1.0	88.8
accD-psaI_p2	TGCTTACATTGCATTTGCGG	ATACGTTCTGATCGCCAACC	45.0	50.0	58.0	58.1	1210-1336 bp	1.0	87.7
accD-psaI_p3	GTATGTGCTTCTGGAGGAGC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1146-1273 bp	1.0	87.6
accD-psaI_p4	TGCTTACATTGCATTTGCGG	CCAGGAAATCCCCAACGAAA	45.0	50.0	58.0	58.1	1519-1645 bp	1.0	86.9
accD-psaI_p5	TGCTTACATTGCATTTGCGG	TTGTTTTTCGAGACCCCGTT	45.0	45.0	58.0	58.0	1245-1371 bp	0.833333	80.9