Hotspot

psaA-ycf3

LSC · 48768-49677 · 910 bp

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Pi 0.0283

MarkerSeek score 80.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 46
pi: 0.0283
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	947-1056 bp	1.0	86.8
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	953-1062 bp	1.0	86.6
psaA-ycf3_p3	AGAAATGACCGGGTTTAGCC	AGCCCTTACCCCTGGTAATT	50.0	50.0	57.9	58.0	965-1074 bp	1.0	86.5
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	978-1087 bp	1.0	86.4
psaA-ycf3_p5	AGAAATGACCGGGTTTAGCC	AGCTATAGCCCTTACCCCTG	50.0	55.0	57.9	58.0	971-1080 bp	1.0	86.3