Hotspot

trnG-GCC-trnfM-CAU

LSC · 43396-43627 · 232 bp

Back to Vachellia

Pi 0.0216

MarkerSeek score 65.20

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 12
pi: 0.0216
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	CTTTGCCAAAGAGAAGACGC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.7	58.0	295-310 bp	1.0	84.3
trnG-GCC-trnfM-CAU_p2	CTTTGCCAAAGAGAAGACGC	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.7	57.8	293-308 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p3	CTTTGCCAAAGAGAAGACGC	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.7	57.8	316-331 bp	1.0	82.6
trnG-GCC-trnfM-CAU_p4	CTTTGCCAAAGAGAAGACGC	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.7	57.8	315-330 bp	1.0	82.6
trnG-GCC-trnfM-CAU_p5	CTTTGCCAAAGAGAAGACGC	TCTCTTTATCGCGGGGTAGA	50.0	50.0	57.7	57.6	344-359 bp	1.0	81.3