Hotspot

trnC-GCA-petN

LSC · 32376-33891 · 1516 bp

Back to Vachellia

Pi 0.0171

MarkerSeek score 66.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1516
variable_sites: 51
pi: 0.0171
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1538-1650 bp	1.0	84.3
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	1599-1711 bp	1.0	82.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1504-1616 bp	1.0	82.6
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	TCTTCCCCATACTACGAGGG	50.0	55.0	57.9	57.6	1589-1701 bp	1.0	82.4
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	1479-1591 bp	1.0	81.7