Hotspot

psbI-trnS-GCU

LSC · 9183-9753 · 571 bp

Back to Vachellia

Pi 0.0393

MarkerSeek score 81.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 571
variable_sites: 41
pi: 0.0393
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CCTATCTAACGATCCAGGACG	TGGACTAAAGCGTCGGATTG	52.4	50.0	57.2	58.0	611-691 bp	1.0	74.2
psbI-trnS-GCU_p2	CCTATCTAACGATCCAGGACG	GTGGACTAAAGCGTCGGATT	52.4	50.0	57.2	58.0	612-692 bp	1.0	74.2
psbI-trnS-GCU_p3	CCTATCTAACGATCCAGGACG	TTTTTCGTACCGAGGGTTCG	52.4	50.0	57.2	58.2	574-654 bp	1.0	72.8
psbI-trnS-GCU_p4	CCTATCTAACGATCCAGGACG	CAATTGGGGAGAGATGGCTG	52.4	55.0	57.2	58.3	633-713 bp	1.0	71.6
psbI-trnS-GCU_p5	CCTATCTAACGATCCAGGACG	GACTAAAGCGTCGGATTGCT	52.4	50.0	57.2	58.4	609-689 bp	1.0	71.4