Hotspot

trnK-UUU-rps16

LSC · 4358-5833 · 1476 bp

Back to Vachellia

Pi 0.0329

MarkerSeek score 63.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1476
variable_sites: 81
pi: 0.0329
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GGGCCCGATCCAATAACAAT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.0	58.0	1378-1872 bp	1.0	88.1
trnK-UUU-rps16_p2	GGGCCCGATCCAATAACAAT	TGAGGTTGCGAATACGAGTG	50.0	50.0	58.0	58.0	1728-2220 bp	1.0	87.6
trnK-UUU-rps16_p3	CCTTTCTGGATCAGTCGTGG	TGAGGTTGCGAATACGAGTG	55.0	50.0	58.0	58.0	1655-2147 bp	1.0	87.6
trnK-UUU-rps16_p4	GGGCCCGATCCAATAACAAT	AAGCTAACGGATCGTGGAAC	50.0	50.0	58.0	58.0	1934-2418 bp	1.0	87.3
trnK-UUU-rps16_p5	CCTTTCTGGATCAGTCGTGG	AAGCTAACGGATCGTGGAAC	55.0	50.0	58.0	58.0	1861-2345 bp	1.0	87.3