Hotspot

rrn16-rps16

Genome · 169693-172501 · 2809 bp

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Pi 0.109

MarkerSeek score 78.60

Primer available yes

Label rrn16-rps16

Feature Metadata

label_name: rrn16-rps16
feature_type: igs
region: Genome
length_bp: 2809
variable_sites: 834
pi: 0.109
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-rps16_p1	CCAGGATCGAACTCTCCATG	TGCTCAACCTACAGAAACGG	55.0	50.0	57.5	57.8	1601-2980 bp	0.857143	79.6
rrn16-rps16_p2	CCAGGATCGAACTCTCCATG	GCTCAACCTACAGAAACGGT	55.0	50.0	57.5	57.8	1600-2979 bp	0.857143	79.6
rrn16-rps16_p3	TCCGACTTGCATGTGTCAAG	TGCTCAACCTACAGAAACGG	50.0	50.0	58.5	57.8	1645-2979 bp	0.714286	74.1
rrn16-rps16_p4	TCCGACTTGCATGTGTCAAG	GCTCAACCTACAGAAACGGT	50.0	50.0	58.5	57.8	1644-2978 bp	0.714286	74.1
rrn16-rps16_p5	CACTGGAAACACCACTTCCC	TGCTCAACCTACAGAAACGG	55.0	50.0	58.7	57.8	1666-3000 bp	0.714286	72.5